Extended Data Fig. 7: Supporting details for in vitro validation of predicted CDR3β sequences.
From: Conditional generation of real antigen-specific T cell receptor sequences
(a) Detailed schematic of Gibson cloning generated CDR3β sequences into a retroviral expression plasmid containing a WT1 TCR sequence, retrovirus generation using 293T cells, and retroviral transduction to generate TCR-Jurkat cell lines for validation studies. (b) Gating strategy used to assess TCR expression. (c) Raw relative luminescence units (RLUs) for 40 generated CDR3β sequences stimulated with WT1 peptide or DMSO (n = 3 technical replicates). Error bars show SEM. Panel ‘a’ created in BioRender.
