Fig. 6: In vitro validation of TCRT5-generated CDR3β sequences.
From: Conditional generation of real antigen-specific T cell receptor sequences
a, Schematic depicting the generation and functional validation of NFAT-luciferase reporter Jurkat cells containing the predicted CDR3β sequences. b, Expression of TCRαβ on CD8+ Jurkat cells following retroviral transduction (n = 3 technical replicates). c, Fold change of RLUs for the engineered Jurkat cells co-cultured with WT1 peptide-pulsed versus DMSO control-treated T2 cells (n = 3 technical replicates). d, Follow-up functional validation assay of RLU fold change for WT1 and F8 Jurkat cells (n = 12 technical replicates). e, Specificity assay. Fold change of RLUs for WT1 and F8 Jurkat cells following co-culture with WT1 peptide, minor histocompatibility antigen HA-1 or CEFX Ultra SuperStim versus DMSO-treated T2 cells (n = 12 technical replicates). All tests of significance were done using the Student’s t-test. Error bars for biological samples in b–e show the standard error of the mean. Var, variable; NS, not significant. ****P ≤ .0001, **P ≤ .01. Panel a created with BioRender.com.
