Extended Data Fig. 1: Thermostability measurements of the remaining antibodies and nanobodies.
From: An adaptive autoregressive diffusion approach to design active humanized antibodies and nanobodies
a, Thermal stability profiles of four humanized antibodies compared with the parental 2B04. The melting temperature (Tm) of 2B04 is 68.5 ∘C. Humanized variants 2B04-hAb1.1, 2B04-hAb1.4, and 2B04-hAb1.5 exhibit comparable thermostability, with Tm values of 67.0 ∘C, 67.6 ∘C, and 66.1 ∘C, respectively. The 2B04-hAb1.3 variant displays a lower Tm of 62.8 ∘C. b, Thermal stability profiles of two humanized nanobodies compared with the parental 3-2A2-4. The melting temperature (Tm) of 3-2A2-4 is 56.8 ∘C. Humanized variants 2A2-hNb1.1 and 2A2-hNb1.2 exhibit lower thermostability, with Tm values of 52.9 ∘C and 50.2 ∘C, respectively.
