Fig. 2: Manipulation of nucleotide sugar biosynthetic pathways modulates HMO profiles in planta. | Nature Food

Fig. 2: Manipulation of nucleotide sugar biosynthetic pathways modulates HMO profiles in planta.

From: Engineered plants provide a photosynthetic platform for the production of diverse human milk oligosaccharides

Fig. 2

a, HMO biosynthetic pathway expressed for the production of LNFPI. b, Nucleotide sugar biosynthetic pathways expressed with LNFPI pathway. c, Quantification of LNFPI production through expression of LNFPI biosynthetic pathway alongside combinatorially expressed nucleotide sugar biosynthetic pathways using an internal calibration curve obtained with an Agilent 6530 Accurate-Mass Q-ToF MS. The middle bar represents the median. Upper and lower whiskers correspond to the largest and smallest values within 1.5 × the interquartile range, respectively. Upper and lower hinges represent the third and first quartiles, respectively. Statistical analysis was conducted using a heteroscedastic two-tailed Student’s t-test with the LNFPI pathway expressed alone used as the reference group. *P < 0.05. P values are: LNFPI + fucose, 0.030; LNFPI + GlcNAc, 0.012; LNFPI + fucose + GlcNAc, 0.01; LNFPI + GlcNAc + Gal, 0.01; LNFPI + fucose + GlcNAc + Gal, 0.043. A sample size of three leaves was used for each experiment. d, Effect of nucleotide sugar biosynthetic pathway overexpression on HMO profile produced using the LNFPI pathway. Values reflect normalized peak area. Hexose, HexNAc, deoxyhexose (Deoxyhex) determined using m/z and MS/MS fragmentation. We performed mass spectral analysis on an Agilent Q-ToF MS.

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