Extended Data Fig. 5: Knocking out OsLRR2 influences transcript levels of defence-related WRKYs and genes related to phytohormone pathways but not salicylic acid levels in rice.
a−f) The transcript levels of defence-related WRKYs, OsWRKY53 (a), OsWRKY62 (b), OsWRKY70 (c), OsWRKY89 (d), OsWRKY30 (e), and OsWRKY33 (f) in ko-lrr2 and WT plants 0, 3, 8 and 12 h post infestation by gravid brown planthopper (BPH) females. g−n) The transcript levels of genes related to JA- (OsAOS1 (g), OsOPR3 (h), OsJAZ8 (i), and OsPR10a (j)), ABA- (OsNCED3 (k) and OsABI5 (l)), and ethylene- (OsACS2 (m) and OsEBF1 (n)) mediated signalling pathways in ko-lrr2 and WT plants 0, 3, 8 and 12 h post infestation by gravid BPH females. o) Levels of salicylic acid (SA) in ko-lrr2 and WT plants 0, 8, 24 and 48 h post infestation by gravid BPH females. Key: FM, fresh mass. Data in (a−o) are presented as mean values + s.e.m., n = 5 biological replicates, except for kolrr2-1 lines at 12 h post infestation in (c) (n = 4 biological replicates). Asterisks in (a−n) indicate significant differences in ko-lrr2 lines at indicated times compared with WT plants evaluated by Bayesian analysis of variance (*P < 0.05, **P < 0.01, and ***P < 0.001). For P values, see source data. Experiments were repeated twice with similar results.
