Extended Data Fig. 4: PAK4 depletion impact on nuclear protein β-catenin and WNT signalling activity.
a, Negative control for the Topflash experiment using the Fopflash luciferase vector which contains a mutated version of the TCF/LEF binding motifs. There are no changes in Fopflash activity upon stimulation with Wnt-3a ligand for 8 hours in any of the tested cell lines (n= 3 per group) (P > 0.05 for all comparisons). b, Baseline WNT activity levels assessed by Topflash assay (n= 3 per group). Values were normalized to B16 WT CC cell lines and no significant WNT activity changes were observed between PAK4 WT and KO cell. c, Immunoblots for nuclear β-catenin protein levels show no differences between B16 WT CRISPR control, PAK4 KO and PAK4 rescue cells. Results are representative from three independent experiments. Means +/- SEM two-tailed unpaired t-test a, b. Unprocessed blot images and raw data are provided as a Source Data file a-c.
