Extended Data Fig. 1: Additional phenotypic analyses of NPK^EYFP prostate tumors.

a, Representative bright field and ex vivo fluorescence images of prostate, lung, and bone (spine) from NP^EYFP mice (n = 35), NPK^EYFP mice without bone metastases (n = 59), and NPK^EYFP mice with bone metastases (n = 47). b–j, Comparison of NPK^EYFP mice with (n = 47) or without (n = 59) bone metastasis. See also Supplementary Table 1. b, Overall survival; p-value calculated using a two-tailed log-rank test. c, Bladder obstruction; p-value calculated using a two-sided Fisher’s exact test. d–g, Dot-plots showing tumor weight (d) and metastatic load (number of metastases/mouse) to lungs (e), liver (f) and brain (g). p-values were calculated by two-tailed Mann-Whitney test, center-lines show the mean and error bars depict SD. h, Distribution of metastases to specific bone types in NPK^EYFP mice at the time of euthanasia. Shown is the mean with standard deviation; n = 106 mice (n = 59 without bone metastases and n = 47 with bone metastases). i, j, Longitudinal analysis of micro-metastasis in non-terminal mice dissected at the ages shown (n = 26). i, Bar graphs showing the percentage of mice with micro-metastasis at 3 months (n = 7), 4 months (n = 17) and 5 months (n = 2). j, Bar graphs showing the percentage of mice with bone or lung micro-metastases (n = 26).