Extended Data Fig. 6: Comparison between the epiCMIT mitotic clock and the Horvath aging clock.

a, Correlations among epiCMIT, age and Horvath-predicted age in normal and neoplastic B cells. Samples sizes are: NBC, n=10 and MBC, n=9 donors; C1 MCL, n=40; C2 MCL, n=17; n-CLL, n=159; i-CLL, n=69; m-CLL, n=260; GCB DLBCL, n=20 and ABC DLBCL, n=28 patients. R and p-value are derived from linear models. Shaded areas represent 95% confidence intervals. b, epiCMIT and Horvath clocks do not have any CpG in common. CpGs of the Horvath model are divided into positively associated with age (gain of methylation) and negatively associated with age (loss of methylation). In addition, they are further classified into B-cell related or B-cell independent if they are extensively modulated or not during normal B-cell differentiation. Barplots represent single data values. c, The CpGs used to build the epiCMIT and Horvath clock show distinct genomic locations. Barplots represent single data values. d, DNA methylation levels of the CpGs from the epiCMIT and Horvath clocks in normal and neoplastic B cells. Sample sizes are the same as in Fig. 4a. e, The CpGs associated with the epiCMIT and Horvath clocks are located in markedly different chromatin states. Sample sizes are the same as in Fig. 4c. f, Genes associated with epiCMIT and Horvath CpGs show distinct transcriptional states in normal and neoplastic B cells. Gene probes shared across all normalized matrices from normal and neoplastic B cells were retained and were the following: epiCMIT-hyper, n=60; epiCMIT-hypo, n=327; Age positive B-cell related, n=44; Age positive B-cell independent, n=118; Age negative B-cell related, n=49; Age negative B-cell independent, n=101. For the box plot, center line, box limits, whiskers and points represent the median, 25th and 75th percentiles, 1.5x interquartile range and individual samples, respectively. Sample size are the same as in Fig. 4e.