Extended Data Fig. 1: T cell responses to NY-ESO-1 versus durability, NY-ESO-1 status and disease recurrence.
a, T cell responses remain durable in cohort 1 subjects for several months. IFNγ, TNFα, and IL-2 cytokine production by CD8+ T-cells (lower panel) and CD4+ T cells (upper panel) were determined by ICS. (N = 2 cohort 1, black) and N = 2 cohort 2, white). b, Baseline T cell responses vs NY-ESO-1 status. 43/60 subjects had tumor biopsies tested for NY-ESO-1 expression and 8 of these 43 subjects had tumors which expressed NY-ESO-1. The only three subjects which showed high baseline T-cell responses to NY-ESO-1 were from the group of eight subjects which had a positive NY-ESO-1 tumor status. (Ungrouped analyses: NY-ESO-1pos: N = 8 subjects; NY-ESO-1neg: N = 35 subjects; Not Stained: N = 17 subjects). (By Cohort analyses: NY-ESO-1pos: N = 3 Cohort 1, N = 5 Cohort 2; NY-ESO-1neg: N = 17 Cohort 1, N = 18 Cohort 2; Not Stained: N = 10 Cohort 1, N = 17 Cohort 2. Two-sided t-test). c, Overall T cell responses vs disease recurrence. Cohort 1 showed greater elevation in anti-NY-ESO-1 T-cell responses than cohort 2. This higher antigen-specific response, however, did not translate to lower levels of recurrence in cohort 1 (12/30 cohort 1 subjects experienced recurrence, vs 8/30 in cohort 2). Statistics for non-recurrence (NR) vs recurrence (R) within each cohort were calculated by a 2 tailed t-test. For cohort 1, n = 18 NR subjects vs n = 12R subjects. For cohort 2, n = 21 NR subjects vs n = 9R subjects. b,c, Bars indicate mean±SD.
