Extended Data Fig. 3: DC subset gating and fold increase of DC subsets during Flt3L mobilization. | Nature Cancer

Extended Data Fig. 3: DC subset gating and fold increase of DC subsets during Flt3L mobilization.

From: Flt3 ligand augments immune responses to anti-DEC-205-NY-ESO-1 vaccine through expansion of dendritic cell subsets

Extended Data Fig. 3

a, Flow cytometry gating strategy; To determine different DC subsets, gating was performed as follows: Lineage- Blue+ HLA-DR+ CD45RA- CD123- CD11c+ (CD11c+), gating on CD11c+ →CD141+ Clec9a+(DC1), gating on CD11c+ →CD141-Clec9a- CD1c+ (DC2), gating on CD11c+ →CD141-Clec9a- CD1c− CD16+(DC4), gating on Lineage- Blue+ HLA-DR+→CD123+CD45RA+ (DC6), gating on Lineage- Blue+ HLA-DR+ CD123+ CD45RA+ CD33+ AXL- (pre-DC). b, Mean fold increases of the indicated DC subsets from each cohort (analyses of n = 7 independent subjects per cohort)(Two-sided t-test).

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