Extended Data Fig. 7: SPI1-rearrangement is associated with developmental arrest and related to dasatinib sensitivity in T-ALL.
a, Dose-dependent cell death was determined for dasatinib using the ex vivo drug sensitivity assay as described in Methods. For each patient at each drug concentration, cells were tested in duplicates. b, Mouse Lin-Sca+Ckit + (LSK) cells were isolated from bone marrow and transduced lentivirally with SPI1 fusion gene or empty vector. After in vitro differentiation in the presence of OP9-DL1 cells and Il7 and Flt3 ligand, LSK cells were subjected to flow-cytometry analysis. TCF7-SPI1 expressing cells exhibited differentiation blockade at DN stage while the empty control cells were able to extensively differentiate to double positive and single positive stages.
