Extended Data Fig. 8: FACS analysis of tumors treated with ionizing radiation.
a, FACS gating scheme for live dead analysis in established tumors. E0771 cells (5x104) were orthotopically injected into WT C57BL/6 J mice. The tumors were treated with IR (8 Gy) when they reached 100 ± 20 mm3 and harvested and analyzed by FACS 24 h after IR. For caspase activity, a single cell suspension was incubated for 1 h with the FAM-FLICA Poly Caspase substrate before FACS stain and analysis. Mean ± SD, unpaired two-tailed t test with Welch’s correction. b, FACS gating scheme for experiments in Fig. 6c,d
