Extended Data Fig. 9: 4T1-luc Enpp1−/− tumor growth.
a, Validating Enpp1−/− 4T1-luc clones (11 clones were pooled) using the 32P-cGAMP degradation assay (3 day incubation). Lysates were normalized by protein concentrations. Data are from 1 experiment, representative of three independent validations; 2 technical protein concentration replicates are plotted with a bar representing the mean. b, Established 4T1-luc WT (harboring scrambled sgRNA) (n = 10 mice) or Enpp1−/− tumors (n = 10 mice) (100 ± 20 mm3) were monitored without treatment. Tumor volumes for individual mice are shown. c, Established 4T1-luc WT (harboring scrambled sgRNA) (n = 10 mice) or Enpp1−/− tumors (n = 10 mice) (100 ± 20 mm3) were treated with IR (20 Gy) and monitored. Tumor volumes for individual mice are shown. d, Established 4T1-luc WT (harboring scrambled sgRNA) (n = 9 mice) or Enpp1−/− tumors (n = 9 mice) (100 ± 20 mm3) were treated with three intratumoral injections of 10 µg cGAMP on day 2, 4, and 7 and monitored. Tumor volumes for individual mice are shown.
