Extended Data Fig. 6: NetBID identifies regulatory nodes of prednisolone resistance.

a, Enrichment of previously reported resistance genes (n = 40 genes and miRNAs; Wilcoxon two-tailed p-value) in NetBID results. b, Heatmap of top 48 NetBID-predicted drivers (‘symbol’_’regulon size’) are ranked by integrated NetBID p-value. Left: color-coded by z-score and labeled by p-value of NetBID results in TOTXVI, TOTXV, and combination (Comb); Right: differential expression of each driver itself, color-coded by z-score and labeled by signed fold-change in TOTXVI, TOTXVI and combination (Comb; (Stouffer’s combined Bayesian generalized linear model “NetBID” p-value; n = 203 patients). c, CELSR2 regulon from B-ALLi (n = 399 genes). Legends of node and edge follow Fig. 6c. d, Enrichment of NetBID-inferred CELSR2 regulon (n = 399 genes) in differentially expressed genes of CELSR2 knockdown vs. NTC in Nalm-6 human ALL cell lines (n = 222 genes; Wilcoxon two-tailed p-value) upon prednisolone treatment for 24 hr (top) Blue lines inside the box indicate the down-regulation of CELSR2 itself, labeled p-value and signed fold-change. e, Enrichment of previously reported resistance genes (n = 40 genes and miRNAs; Wilcoxon two-tailed p-value) in differentially expressed genes of CELSR2 knockdown vs. NTC in NALM-6 ALL cell lines without prednisolone treatment.