Extended Data Fig. 5: Impact of STAU2 knockdown on human myeloid leukemia and normal human HSPCs.
a, Graphs show relative STAU2 expression in K562 cells transduced with either LacZ (control) or STAU2 shRNAs (A and B; n = 3 technical replicates per group). b, Relative STAU2 expression in two representative primary human myeloid leukemia samples transduced with shLacZ or shSTAU2. Expression was analyzed 72 h post transduction (n = 3 technical replicates per group). c, Representative FACS plot (left) and graph (right) showing the frequency of CD34+ cells in engrafted human leukemia samples in the bone marrow of NSG mice 7-8 weeks post-transplant. Each dot represents a mouse and each color represents a primary human sample (bcCML in blue and AML in red; mean±S.E.M.; n = 4 animals per cohort). d, Representative FACS plot (left) and graph (right) showing the frequency of CD11b+ differentiated cells in the engrafted human leukemia samples in the bone marrow of NSG mice 7-8 weeks post-transplant. Each dot represents a mouse and each color represents a primary human sample (bcCML in blue and AML in red; mean±S.E.M.; n = 4 animals per cohort). e, Representative FACS plot (left) and graph (right) showing the relative frequency of CD34+ CD38− cells in the engrafted normal primary human CD34+ HSPC samples in the bone marrow of NSG mice 8 weeks post-transplant. Each dot represents a mouse and each color represents an individual human sample (mean±S.E.M.; n = 4 animals per cohort).
