Extended Data Fig. 4: Depletion of macrophages and T cells does not selectively increase monoclonal metastasis.
a, Treatment schematic and representative flow plots of lung-resident macrophages from bronchoalveolar lavage fluid of Ctrl liposome or clodrosome-treated mice (n = 3 mice). b, Lung metastasis growth curve and representative whole body mouse bioluminescence images of mice injected with single cells or clusters of AT3 cells following intranasal treatment with ctrl liposome or clodrosome (n = 5 mice). c, Treatment schematic and representative flow plot of blood T cells from Ctrl, anti-CD4 and anti-CD8 antibody treated mice. (n = 3 mice). d, e, Quantification of total lesions number and single/ mixed-colored lung metastases from wildtype mice treated with control, anti-CD4, or anti-CD8 antibody as absolute lesions counts (left and middle) or percentages of total (right) (Ctrl, n = 9; anti-CD4, n = 10; anti-CD8, n = 10 mice). Mean ± s.d. d, e, or s.e.m. (b) shown. P values were calculated by two-tailed unpaired Mann–Whitney U-test (d, e) or two-way repeated measures ANOVA (b).
