Extended Data Fig. 5: NK cell depletion attenuates the metastatic advantage of disseminated tumor cell clusters.
a, Treatment schematic and representative flow plots of blood NK cells in Rag2yc-dKO mice following NK cell adoptive transfer (Representative of n = 3 mice). b, Primary tumor volume of Rag2yc-dKO mice with or without NK cell adoptive transfer. c, Quantification and representative images of lung bioluminescence (as estimate of tumor burden) in Rag2yc-dKO mice following NK cell adoptive transfer (For b, c, Rag2yc-dKO, n = 11 mice; Rag2yc-dKO + NK, n = 10 mice). d, e, Lung metastasis growth curve and representative whole body mouse bioluminescence images of mice injected with single cells or clusters of e) AT3 cells and f) 4T1 cells following treatment with ctrl antibody or anti-asialo GM1 (n = 5 per group). (Mean ± s.d.(b, c) or s.e.m. d, e, shown. P values were calculated by two-tailed unpaired Student’s t-test (b), two-tailed unpaired Mann–Whitney U-test (c), or two-way repeated measures ANOVA.
