Extended Data Fig. 7: Epithelial-to-mesenchymal transition alters cluster formation and expression of NK cell ligands.
a,b, Zeb1 expression levels (measured by qRT-PCR) and representative microscopy images of AT3 ctrl and miR-200c cells, T11 ctrl and miR-200c cells and PyMT-M and -E cells. (AT3, PyMT, n = 3; T11, n = 5 independent experiments) Scale bar, 100 µm. c, In vitro cell proliferation assay showing relative areas of T11 Ctrl and T11 miR-200c, and AT3 Ctrl and AT3 miR-200c. (n = 3 independent experiments). d, Representative microscopy images showing the invasive edge of AT3 tumor organoids from Ctrl and miR-200c cells cultured on Matrigel. Arrowheads show invasive single cells and arrows show collectively migrating clusters. (n = 5 organoids) Scale bar, 50 µm. e, Representative microscopy images of T11 ctrl and T11 miR-200c cells in suspension. Scale bar, 100 µm (n = 5 images/cell line). f, (Left) Gating strategy and histogram of flow cytometry analysis of Rae1 in T11 miR-200c, AT3 miR-200c, PyMT-E and PyMT-M cell lines. (Right) The fold change in Rae1 median fluorescent intensity (MFI) of PyMT-E cells over PyMT-M is shown. (n = 3 independent experiments). Mean ± s.e.m. shown. P values were calculated by two-tailed paired Student’s t-test (a, paired by batch), two-way repeated measures ANOVA (c), or one sample t test (f). N/A, not applicable.
