Extended Data Fig. 8: Correlations between EMT characteristics, expression of NK cell ligands and cancer cell clustering in pre-clinical and clinical data.
a, EMT and NK cell activating gene signature scores calculated based on RNA-seq data of non cluster-forming and cluster-forming breast cancer cell lines (from GSE104765, n = 3 technical replicates/ cell line). b, (left) The correlation between NK cell activating score, and observed NK cell-mediated killing of cancer cells in vitro (right) Correlation between NK cell activating score and EMT score of cluster-forming and non cluster-forming cell lines (GSE104765, n = 3 technical replicates/ cell line). c, EMT and NK cell activating score of MCF10A cells with or without TGF-β treatment (from GSE81955, n = 3 independent experiments). d, EMT score of single and cluster CTCs isolated from breast cancer patients (from GSE51827, n = 15 pools of single CTCs and 14 CTC-clusters isolated from 10 breast cancer patients). (Mean ± s.e.m. shown. Pearson’s correlation coefficient was shown (b). P values were calculated by two-tailed unpaired Student’s t-test (a,c,d). For boxplots (a, d), the center shows the median; Upper and lower hinges show the first and third quartiles; and the upper and lower whiskers show highest and lowest values within 1.5 × IQR (interquartile range) of the hinge.
