Extended Data Fig. 3: Zeb2IEC-Tg/+ mice develop severe pathology in colon but not in small intestine.
From: Zeb2 drives invasive and microbiota-dependent colon carcinoma
a, Quantification of Ki67-, CD44-, P-Stat3- and P-Smad3-positive cells in different regions of the colon (mucosa, submucosa, muscle or serosa) of 25-30-week old wild-type (WT) and Zeb2IEC-Tg/+ mice (Tg) (Ki67 Tg/+, n=9 mice and WT, n=6 mice; CD44 Tg/+, n=5 mice and WT, n=5 mice; P-Stat3 Tg/+, n=7 mice and WT, n=5 mice; P-Smad3 Tg/+, n=6 mice and WT, n=3 mice). For each tissue region and mouse, 3 separate areas were analyzed and averages per mouse per region were plotted in GraphPad Prism. Data are presented as mean ± SEM. ** p<0.01, *** p<0.001, **** p<0.0001. Two way ANOVA, corrected for multiple comparisons using Sidak test in GraphPad Prism. b, Representative FACS plots of Ki67+EPCAM+ cells in colon (left panel) and small intestine (right panel) in 5 week old wild-type (WT) and Zeb2IEC-Tg/+ mice. The experiment was performed twice with similar results. c, Quantitative PCR analysis for Zeb2 and eGFP in lysates from the small intestine of Zeb2IEC-Tg/+ (Tg/+, n=6 mice) and wild-type littermate controls (WT, n=5 mice). Data are presented as mean ± SEM and were analyzed with two-tailed Mann Whitney test, p-value = 0.0043 for both. d, Quantification of Ki67-positive cells in different regions of the small intestine of 25-30-week old wild-type (WT, n=4 mice) and Zeb2IEC-Tg/+ mice (Tg, n=5 mice). For each tissue region and mouse, 3 separate areas were analyzed and averages per mouse per region were plotted in GraphPad Prism. Data are presented as mean ± SEM and were analyzed with two-way ANOVA with Sidak correction. e, Quantitative PCR analysis for inflammatory markers (TNF, IL-1β, Cox2, Cxcl5 and A20), CD44, and Paneth cell markers (Crypt-1 and LysP) in lysates from the small intestine of Zeb2IEC-Tg/+ (Tg/+, n=6 mice) and wild-type littermate controls (WT, n=5 mice). Data are presented as mean ± SEM and were analyzed with two-tailed Mann Whitney test, p-value = 0.0087 for IL-1β and 0.0043 for Cox2; ns, non-significant.
