Extended Data Fig. 4: Tumorigenic potential and copy number profiling of Zeb2 transgenic tissue.
From: Zeb2 drives invasive and microbiota-dependent colon carcinoma
a, Orthotopic transplantation of intestinal tumor cells from Zeb2 transgenic mice into the cecum of NSG mice. Zeb2 transgenic cells could be identified by immunohistochemical staining for the GFP transgene. Scale bar: 200µm; boxed area Scale bar: 100µm. Data representative for one experiment on 5 NSG mice. b, Copy number analysis by shallow whole genome sequencing of Zeb2IEC-Tg/+ colon tumor tissue (n=4 mice, lanes 3-6), normal lung tissue (n=2 mice, lanes 1-2) and normal colon tissue from a wild-type littermate control mouse (lane 7). Genomic aberrations are positioned along the murine chromosomes and visualized in red for deletions and blue for amplifications.
