Extended Data Fig. 8: Microbiota dysbiosis in Zeb2^IEC-Tg/+ mice.

a, b, Altered bacterial diversity/community structure in mucosal samples from 25 week-old wild-type (WT, in green, n=28 mice) and Zeb2^IEC-Tg/+ (Tg/+, in red, n=17 mice) mice. α-diversity measured by the Shannon Diversity Index at the genus level (p= 0.1439, Welch two Sample t-test) (a). β-diversity represented by a PCoA of Bray-Curtis dissimilarity distances of bacterial composition (b). c, d, Differentially abundant taxa between wild-type (WT) and Zeb2^IEC-Tg/+ (Tg/+). Linear Discriminant Analysis Effect Size (LEfSe) cladogram of differentially abundant taxa in mucosal samples from WT (in green, n=21 mice) and Tg/+ (in red, n=34 mice) mice (c). Linear Discriminant Analyis (LDA) scores (d) of differentially abundant taxa combined with multiple testing corrections (analysis associated with Extended Data Fig. 8c): t-test with FDR correction (*: padj <0.05) (no significant differences were identified based on MaAslin analysis). [phylum (p), class (c), order (o), family (f), genera (g), species (s)]. e, Abundance of significantly different taxa between wild-type (WT) and Zeb2^IEC-Tg/+ (Tg/+) mucosal samples based on t-test FDR correction analysis. The horizontal straight line indicates the group means and the dotted line indicates the group medians. f, g, Mycobiota profiling in Zeb2^IEC-Tg/+ mice. Fungal diversity/community (WT n=33 mice, in green, and Tg/+ n=22 mice, in red). α-diversity measured by the Shannon Diversity Index at the genus level (p= 0.0717, Welch two Sample t-test) (f). β-diversity represented by a PCoA of Bray-Curtis dissimilarity distances of fungal composition (g). Each box represents the third (upper) and first (lower) quartiles. Lines inside the boxes represent the median.

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