Extended Data Fig. 1: Expression of Zeb2 in the mouse intestinal epithelium.
From: Zeb2 drives invasive and microbiota-dependent colon carcinoma
a, Schematic representation of the conditional ROSA26-based Zeb2 overexpression mouse model. The mouse Zeb2 open reading frame was targeted to the ROSA26 locus preceded by a floxed (fl) transcriptional stop (PGK-Neo-3XpA) cassette and followed by IRES-EGFP reporter sequence. b, R26-Zeb2Tg/Tg mice were crossed with a Cre transgenic line under the control of Villin promoter, generating R26-Zeb2Tg/+ villin-creTg/+ transgenic (Tg/+) mice and R26-Zeb2Tg/+ villin-cre+/+ (WT) controls. c-d, Kaplan-Meier survival curve (c) and clinical stool score (d) of male (n=12 mice) and female (n=10 mice) Zeb2IEC-Tg/+ mice based on Hemoccult assay. Data are presented as mean ± SEM.
