Extended Data Fig. 6: Gating strategy for FACS analysis of CD4⁺, CD8⁺ and CD8⁺CD44⁺CD62L⁻ T cells in MN/MCA1 sarcomas.

Representative FACS plots showing the gating strategy for FACS analysis of CD4⁺, CD8⁺ and CD8⁺CD44⁺CD62L⁻ T cells in MN/MCA1 tumor samples. Gate of CD4⁺ cells corresponds to FACS data panels of CD4⁺/Aqua⁻ (%) of Fig. 6a,g and Extended Data Fig. 4d. Gate of CD8⁺ cells corresponds to FACS data panels of CD8⁺/Aqua⁻ (%) of Fig. 6e,g and Extended Data Fig. 4d. Gate of CD8⁺CD44⁺CD62L⁻ cells (upper right panel) corresponds to FACS data panels of CD8⁺CD44⁺CD62L⁻/Aqua⁻ (%) of Fig. 6f,g and Extended Data Fig. 4d.