Extended Data Fig. 1: The lectin pathway and the C3aR promote 3-MCA-induced sarcomagenesis and FS6 sarcoma growth.
a−h, 3-MCA-induced sarcoma growth curves (left panels) or mean tumor volume (± SEM, right panels) in C3−/− (a, n = 20 wt, n = 18 ko), MBL1/2−/− (b, n = 10 wt, n = 14 ko), C4−/− (c, n = 10 mice in each group), C1q−/− (d, n = 7 wt, n = 8 ko), fB−/− (e, n = 9 wt, n = 12 ko), C5aR1−/− (f, n = 10 wt, n = 19 ko), C5aR2−/− (g, n = 14 wt, n = 10 ko) and C3aR−/− (h, n = 14 wt, n = 16 ko) mice. Experiment of panel d was performed using wt littermates. i, Representative H&E staining of 3-MCA tumor tissues of wt, C3−/−, MBL1/2−/−, C4−/− and C3aR−/− mice. One experiment performed, three representative fields have been acquired for each group (n = 4 mice in each group). Scale bar: 100 µm. j-q, FS6 tumor incidences in C3−/− (j, n = 4 wt, n = 8 ko); MBL1/2−/− (k, n = 10 wt, n = 9 ko); C4-/ (l, n = 11 wt, n = 10 ko); C3aR−/− (m, n = 15 wt, n = 12 ko); C1q−/− (n, n = 15 wt, n = 8 ko); fB−/− (o, n = 9 wt, n = 4 ko); C5aR1−/− (p, n = 12 wt, n = 5 ko) and C5aR2−/− (q, n = 12 wt, n = 8 ko) mice. r-u, FS6 tumor volumes (mean ± SEM) (r, t) and incidences (s, u) in MBL1/2±/- (r-s, n = 12 he, n = 8 ko) and C3aR±/- (t-u, n = 21 he, n = 12 ko) littermates. One representative experiment out of three (a), two (b, d, e, f, j and k) or one (c, g, h, l, n, p, q, r, s, t and u) performed is shown. o, m: two pooled experiments. The same wt mice were used simultaneously as control mice of experiments of panels p, q and one out of the two pooled experiments of panel m. Two-tailed Mann Whitney test (r) or unpaired two-tailed Student’s t test (t). Exact p values are reported, two-tailed Wilcoxon matched-pairs signed rank test (j-q, s and u).
