Extended Data Fig. 3: LKB1 mutant tumor double strand break repair.
a, HR and NHEJ levels in Kras/Lkb1/Trp53 mutant cell lines comparing with Kras/Trp53 cells. n = 3 cell cultures for each group. (mean±sd, two-sided t test, unpaired). b, NHEJ ratio changes in human NSCLC LKB1 mutant cell lines H23, A427 and H460 with empty vector (pBABE), LKB1 or KLB1-KD overexpression were determined by flow cytometry. n = 3 individual cell cultures for each group. (mean±sd, two-sided t test, unpaired). c, Quantification of Rad51 and pH2AX positive cells percentage in KLP cells. Result is combined from three independent experiments. KP UT, KP NCS and KLP UT n = 4, KLP NCS n = 3 individual cell cultures each group. Data shown one of two independent experiments. (mean ± sd, one-way ANOVA, multiple two-sided comparison, Tukey test). d, LKB1 forms complex with BRCA1 in response to DNA damage. Upper panel, immunoprecipitation assay showing LKB1 interacting proteins in response to neocarzinostatin (NCS) treatment induced DNA damage. Lower panel, western blot showing total level of input proteins from whole-cell extract (WCE). Data represents one of two independent experiments. Blots are cropped and uncropped images can be found in Source Data.
