Extended Data Fig. 2: IL-8 Regulation in Prostate Tumor Epithelial Cells.
From: Castration-mediated IL-8 promotes myeloid infiltration and prostate cancer progression
a, Percentage input bound in ChIP-qPCR assays assessing binding of AR, pSer5 Pol II, and H3K9ac at the KLK3 (PSA) promoter (top) and upstream region (bottom) in LNCaP cells cultured at indicated concentrations of DHT for 8hrs and TNFα (50Units/ml) for 6hrs, cells cultured in androgen-free media for 72hrs before DHT stimulation (n = 2 independently cultured replicates per group). b, ChIP-Seq analysis of AR and NF-κB p65 subunit at the IL-8 (CXCL8) promoter in LNCaP cells cultured in the presence of either vehicle (DMSO), DHT (100 nM), or TNFα (1000U/ml) (n = 2 replicates per group; GSE83860). c, ChIP-Seq analysis at the IL-8 (CXCL8) promoter for AR and RNA pol II binding in LNCaP cells (top), and AR binding in VCaP cells (bottom). Both cell lines were cultured in the presence of either vehicle (DMSO) or DHT (10 nM) (GSE55064). For b, loci with significant differential binding (black bar) were identified as described in materials and methods. Blue shading marks AR binding site; while orange shading marks NF-κB p65 subunit binding site. Bar plots represent means with SEM. Unpaired one-tailed t-tests were performed, p-values ≤ 0.0001 (****); p-values ≥ 0.05 (ns).
