Extended Data Fig. 8: ESK981 induces autophagosome formation and upregulates Cxcl10 expression in various murine cancer cell lines. | Nature Cancer

Extended Data Fig. 8: ESK981 induces autophagosome formation and upregulates Cxcl10 expression in various murine cancer cell lines.

From: Autophagy inhibition by targeting PIKfyve potentiates response to immune checkpoint blockade in prostate cancer

Extended Data Fig. 8

a, IC50 from cell viability assays of ESK981 in murine cancer cells of lung (Ae17, LLC), melanoma (B16F10), ovarian (ID8), pancreas (PAN02), renal (Renca), prostate (TRAMP-C2), and breast (4T1) lineages. Data were plotted as mean ± SEM from three independent experiments. b, Autophagosome content measured by CYTO-ID in indicated cell lines treated with control (Ctrl) or 300 nM ESK981 for 24 hours. Data were analyzed by two-tailed unpaired t test from four independent experiments and presented as mean ± SEM. P-value indicated. c, mRNA level of Cxcl10 in indicated cell lines treated with 10 ng/ml mIFNγ or mIFNγ plus 300 nM ESK981 for 24 hours. Data were analyzed by two-tailed unpaired t test from three (PAN02, Ae17) or four (ID8, B16F10, Renca, 4T1, TRAMP-C2, LLC) independent experiments and presented as mean ± SEM. P-value indicated.

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