Extended Data Fig. 3: PUS7 regulates GSC growth in a catalytic activity dependent manner.
From: Targeting PUS7 suppresses tRNA pseudouridylation and glioblastoma tumorigenesis
The WT but not the mutant (Mut) PUS7 rescued PUS KD-induced growth inhibition in PBT003 and PBT707 GSCs. n = 4 cell culture replicates. p < 0.0001 for shPUS7 (-) and PUS7 (-) vs shPUS7 (+) and PUS7 (-), p < 0.0001 for shPUS7 (+) and PUS7 (-) vs shPUS7 (+) and WT PUS7 ( + ), ns: p = 0.7179 for shPUS7 (+) and PUS7 (-) vs shPUS7 (+) and Mut PUS7 ( + ) in PBT003; p < 0.0001 for shPUS7 (-) and PUS7(-) vs shPUS7 (+) and PUS7(-), p < 0.0001 for shPUS7 (+) and PUS7 (-) vs shPUS7 (+) and WT PUS7 ( + ), ns: p = 0.9976 for shPUS7 (+) and PUS7 (-) vs shPUS7 (+) and Mut PUS7 ( + ) in PBT707. Error bars are SE of the mean for this figure. ***p < 0.001 and ns: not statistically significant (p > 0.05, defined above) by One-way ANOVA and Dunnett’s multiple comparisons test for this figure.
