Extended Data Fig. 5: PUS7 inhibitors suppress GSC growth.
From: Targeting PUS7 suppresses tRNA pseudouridylation and glioblastoma tumorigenesis
(a) Cell growth of PBT003 GSCs treated with the C4 PUS7 inhibitor. n = 4 cell culture replicates. p = 0.0009 for 10 µM and p < 0.0001 for 50 µM condition. (b) Cell growth of PBT003 GSCs or NSC009 NSCs treated with the C17 PUS7 inhibitor. n = 4 cell culture replicates. p < 0.0001 for PBT003 and ns: p = 0.2831 for NSC009. (c) IC50 test for C17 compound in GSCs (PBT003, PBT707, PBT726, and PBT111). For each GSC, n = 4 cell culture replicates for each treatment condition. (d) Cell growth of GSC (PBT707, PBT726, and PBT111) treated with the C17 analog compound. n = 4 cell culture replicates. p = 0.0002, <0.0001, <0.0001, <0.0001 for 0.4, 2, 10, 50 µM conditions respectively in PBT707; p < 0.0001 for 2, 10, 50 µM conditions in PBT726; p < 0.0001 for 2, 10, 50 µM conditions in PBT111. Error bars are SE of the mean. ***p < 0.001 by One-way ANOVA and Dunnett’s multiple comparisons test for panels a and d. ***p < 0.001 and ns: not statistically significant (p > 0.05, defined above) by one-tailed Student’s test for panel b.
