Extended Data Fig. 7: PUS7 regulates IFN pathway in GSC.
From: Targeting PUS7 suppresses tRNA pseudouridylation and glioblastoma tumorigenesis
(a) Correlation analysis of PUS7 expression and IFN gene signature (IFN alpha response gene signature and IFN gamma response gene signature) analyzed by ssGSEA in GBM patients from the TCGA dataset. (b) The growth of PBT003 and PBT707 GSCs treated with IFNα. n = 4 cell culture replicates. p = 0.0093 for 20 ng/ml and p = 0.0002 for 100 ng/ml in PBT003; p = 0.0064, <0.0001, <0.0001 for 4, 20, 100 ng/ml conditions, respectively, in PBT707. (c) RT-PCR of ISGs in C17 compound-treated PBT003 GSCs. n = 3 technical replicates. p = 0.0041 for ISG15 and p = 0.0015 for XAF1. (d) RT-PCR of ISGs in C17 compound-treated tumor derived from PBT003 GSCs. n = 3 technical replicates. p = 0.0004 for ISG15 and p = 0.0001 for XAF1. Error bars are SE of the mean. **p < 0.01 and ***p < 0.001 by One-way ANOVA and Dunnett’s multiple comparisons test for panels b, and by one-tailed Student’s t-test for panels c and d.
