Extended Data Fig. 9: IRS1 phosphorylation and response to therapy.
(a) Pearson’s correlation determination (r^2) test was used to calculate the correlation coefficient and the p-value between CTP and all detected phosphorylation sites in IRS1 (upper panel) and IRS2 (lower panel). The correlation was calculated based on n = 7 clones as well as the non-clonal PC9 population. FDR < 0.1 (b) pIRS1 (ser1101) and pIRS1 (ser1078) were measured in drug-naïve cells and DRPs of the G361 cell line treated 0.06 µM trametinib. Data are presented as mean values, error bars represent the standard deviation of n = 3 independent cultures. p-values were calculated by one tailed Student’s t-test.
