Extended Data Fig. 2: CIP2A promotes genome integrity of BRCA-deficient cells.
a, b, Colocalization analysis of CIP2A and γH2AX in DLD1 cells pre- and post-IR (2 Gy) using Squassh (size parameter). Quantitation is shown in (a). Threshold for colocalization is 0.5 (dashed line). Data points represent cells analysed and bars represent the mean ± 95% C.I. n = 15 aggregated from 3 independent immunostainings. Representative micrographs are shown in (b). UT, untreated. c, Representative micrographs of the experiment shown in Fig. 3f. d, Quantitation of radial chromosomes (left) and chromatid breaks (right) in metaphase spreads from DLD1 cells upon transduction of virus expressing the indicated sgRNAs along with Cas9. The bars represent the mean ± S.D. n = 30 aggregated from 3 independent experiments with 10 metaphases scored per experiment; analyzed with Kruskall-Wallis non-parametric test, followed by Dunn’s multiple comparisons, compared to sgAAVS1; n.s.=1.0000, ∗p = 0.0107 (radials); ∗∗∗p = 0.0001, 0.0044 (breaks). e, Representative micrographs of the experiment presented in Fig. 3g. Arrowheads indicate chromosome aberrations. f, Representative micrographs of the experiment shown in Fig. 3h. MNi are indicated with white triangles. Scale bar = 10 μm. Statistical analyses are found in Source Data ED Fig. 2.
