Extended Data Fig. 6: Data supporting that the CIP2A-TOPBP1 interaction promotes HR-deficient cell viability.
a, Immunoblots of whole-cell extracts from DLD1 parental (WT) or BRCA2-/- cells transduced with the indicated HA-tagged TOPBP1-expressing lentivirus or an empty virus that expresses an HA epitope (EV(HA)). Lysates were probed with antibodies to HA or tubulin (loading control). FL, full-length. Representative of n = 3 independent transductions. b, Micrographs of DLD1 cells transduced with the indicated virus that were arrested in mitosis with a 16 h treatment with nocodazole, exposed to a 2 Gy IR dose and processed for immunofluorescence with the indicated antibodies 1 h later. Relates to the experiment quantitated in Fig. 5h. Representative of three immunostainings. c, Representative images of crystal violet stains of the clonogenic survival experiment presented in Fig. 5i. Representative of three clonogenic assays. d, Representative micrographs of the experiment presented in Fig. 5j showing DAPI-stained cells to monitor micronucleation (labeled with arrowheads). Representative of three immunostainings. Scale bars =10 μm. Full blots are provided in Source Data ED Fig. 6.
