Extended Data Fig. 1: Supportive Data to Main Fig. 1.
From: A tumor-derived type III collagen-rich ECM niche regulates tumor cell dormancy
All numerical data are presented as mean +/−SEM. (a) Experimental design of CAMs experiments. d refers to days on timeline scheme. Left panel: Representative images of day 6 collected tumors. Right panel: Top graph: T-HEp3 and D-HEp3 (n = 6 independent CAMs). Bottom graph: D2.A1 and D2.OR (n = 5 independent CAMs). Number of cells per tumor compared with an unpaired two-tailed Mann-Whitney test with 95% confidence level. (b) Representative multiphoton images of T-HEp3 and D-HEp3 CAM tumors. Scale bar, 50μm. d refers to days on timeline scheme. (c) Tissue microarray SHG analysis. Left panel: representative images of normal tissue versus stage IV HNSCC ECM architecture. Right images are a zoom of white squares on left image. Scale bars, 200μm. Scale bar zoom, 50 μm. Right panel: Collagen orientation between normal tissues (n =43 samples) and malignant HNSCC (n =289 samples) and between stage I to III (n =130 samples) and stage IV and IVa (n =53 samples). Data were compared using an unpaired two-tailed Mann-Whitney test with 95% confidence level. (d) Imaging window design and implantation site in mice (n =5). Representative images of T-HEp3-GFP in primary site. Scale bar, 100μm. Zoom Scale bar, 50μm. d refers to days on timeline scheme. (e) Left panel: Nude mice lung representative images with or without T-HEp3 GFP spontaneously disseminated cells. Scale bar, 50μm. Right panel: NCG mice lungs representative images with MDA-MB-231 GFP spontaneously disseminated cells. Scale bar, 50μm.
