Extended Data Fig. 7: Gating strategy to flow sort tdTomato-positive brain metastases and DTCs for single cell transcriptomic analysis. | Nature Cancer

Extended Data Fig. 7: Gating strategy to flow sort tdTomato-positive brain metastases and DTCs for single cell transcriptomic analysis.

From: Astrocytic laminin-211 drives disseminated breast tumor cell dormancy in brain

Extended Data Fig. 7

Gating strategy for isolating tdTomato + / mVenus-p27+ micrometastases and tdTomato + / mVenus-p27− macrometastases from brains of NOD-SCID mice inoculated with tdTomato-mVenus-p27K− T4-2 cells via ultrasound-guided intracardiac injection. Dead cells were excluded based on DAPI incorporation. Live cells were further gated off PE-Texas Red (for tdTomato) and FITC-A (for mVenus-p27). Non-inoculated brain cell suspension and tdTomato- or mVenus-p27-expressing T4-2s were used to set gates.

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