Extended Data Fig. 3: The effect of lnc-CTHCC KO in mice HCC and the localization of lnc-CTHCC in single cells from mouse liver cancer.
(a) The expression of adjacent genes, such as MAPK3, GDPD3, INO80E, SEZ6L2, and MVP, in the livers of WT and KO mice (n = 3 mice per group). Data are shown as mean ± SEM. Two-tailed unpaired t-test was performed. (b) Schematic representation of the DEN-induced HCC model. Two-week-old mice were intraperitoneally injected with DEN (25 mg/kg) and then fed a normal diet for 34 weeks (n = 6 mice per group). (c) Representative images of gross morphology from the livers of WT and KO mice. The images are representative of n = 6 animals. (d) The ratio of liver to body weight (P = 0.0003), the number of tumors (P = 0.0001) and the largest tumor diameter (P = 0.0022) from WT and KO mice. (e) ALT and AST levels in the serums of WT and KO mice. P values are as follows: P = 0.0003 (ALT); P = 0.0171 (AST). (f-g) HE, Ki67, TUNEL, and CD31 staining of the livers from WT and KO mice (scale bars = 50 μm). P values are as follows: P < 0.0001 (Ki67); P < 0.0001 (TUNEL); P < 0.0001 (CD31). For D-G, data are shown as mean ± SEM (n = 6 mice per group). Two-tailed unpaired t-test was performed. (h) FISH of lnc-CTHCC in the H22 cell line (scale bars = 5 μm). The results are representative of three independent experiments. (i-j) Liver cancer tissues from WT mice were dissociated into single cells for IF staining and FISH (scale bars = 5 μm). The markers of macrophages, epithelial cells, endothelial cells, and immune cells were F4/80, KRT, CD31, and CD45, respectively. The results are representative of three biologically independent samples. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.
