Extended Data Fig. 6: Lnc-CTHCC has no effect on the expression and localization of hnRNP K.
(a,b) The knockdown efficiencies of hnRNP K at the RNA and protein levels in Hep3B cells. P values are as follows: P = 0.0008 (si-1); P = 0.0004 (si-2); P = 0.0001 (si-3). (c) Overexpression of hnRNP K rescued the colony formation ability of Hep3B cells with lnc-CTHCC knockdown. P values are as follows: P = 0.0004 (sh-NC + NC and sh-lnc-CTHCC + NC); P = 0.0017 (sh-lnc-CTHCC + NC and sh-lnc-CTHCC + hnRNP K). (d) Knockdown of hnRNP K rescued the colony formation ability of SMMC7721 cells with lnc-CTHCC overexpression. P values are as follows: P < 0.0001 (NC + si-NC and lnc-CTHCC + si-NC); P = 0.0006 (lnc-CTHCC + si-NC and lnc-CTHCC + sihnRNP K). (e) Overexpression of hnRNP K rescued the invasion ability of Hep3B cells with lnc-CTHCC knockdown (scale bars = 50 μm). P values are as follows: P = 0.0004 (sh-NC + NC and sh-lncCTHCC + NC); P = 0.0005 (sh-lnc-CTHCC + NC and sh-lnc-CTHCC + hnRNP K). (f) Knockdown of hnRNP K rescued the invasion ability of SMMC7721 cells with lnc-CTHCC overexpression (scale bars = 50 μm). P values are as follows: P < 0.0001 (NC + si-NC and lncCTHCC + si-NC); P = 0.0018 (lnc-CTHCC + si-NC and lnc-CTHCC + si-hnRNP K). (g) The RNA levels of hnRNP K in different HCC cells with knockdown or overexpression of lnc-CTHCC. (h) Western blot assays of hnRNP K in different HCC cells with knockdown or overexpression of lnc-CTHCC. (i) The hnRNP K staining of the xenograft tumors with lnc-CTHCC knockdown or overexpression (scale bars = 50 μm). (j) Cellular immunofluorescence assays of hnRNP K in different HCC cells with knockdown or overexpression of lnc-CTHCC (scale bars = 25 μm). For B and H-J, the results are representative of three independent experiments. For A and C-G, data are from three biologically independent experiments and shown as mean ± SEM. Two-tailed paired t-test was performed. **P < 0.01; ***P < 0.001; ****P < 0.0001.
