Extended Data Fig. 8: YAP is involved in the biological function of lnc-CTHCC in HCC.
(a) The knockdown efficiencies of YAP at the RNA and protein levels in Hep3B cells. P values are as follows: P = 0.0002 (sh-1); P = 0.0001 (sh-2). (b,c) Knockdown of YAP suppressed HCC cell proliferation and metastasis in vitro. (B) Colony formation assays. P values are as follows: P = 0.0034 (sh-YAP-1); P = 0.0057 (sh-YAP-2). (C) Transwell assays. Scale bars = 50 μm. P values are as follows: P = 0.0044 (migration-sh-YAP-1); P = 0.0046 (migration-sh-YAP2); P = 0.0009(invasion-sh-YAP-1); P = 0.0013 (invasion-shYAP-2). (d-f) Knockdown of YAP suppressed lnc-CTHCC-induced increase in HCC cell proliferation and metastasis in vitro. (D) Colony formation assays. P values are as follows: P = 0.0004 (Ctrl + sh-NC and Ctrl + sh-YAP); P = 0.0065 (lnc-CTHCC + sh-NC and lnc-CTHCC + sh-YAP). (E) EdU assays. Scale bars = 50 μm. P values are as follows: P = 0.0117 (Ctrl + sh-NC and Ctrl + shYAP); P = 0.0102 (lnc-CTHCC + sh-NC and lnc-CTHCC + sh-YAP). (F) Transwell assays. Scale bars = 50 μm. P values are as follows: P = 0.0006 (Ctrl + sh-NC and Ctrl + sh-YAP); P = 0.0031 (lnc-CTHCC + sh-NC and lnc-CTHCC + sh-YAP). (g,h) The tumor growth curves and tumor weight (n = 6 mice per group, P < 0.0001). Data are shown as mean ± SEM. Two-tailed unpaired t-test was performed. (i,j) Overexpression of YAP rescued the proliferation and invasion abilities of Hep3B cells with lnc-CTHCC knockdown (scale bars = 50 μm). (I) P values are as follows: P = 0.0012 (sh-NC + NC and sh-lnc-CTHCC + NC); P = 0.0003 (sh-lnc-CTHCC + NC and sh-lnc-CTHCC + YAP). (J) P values are as follows: P = 0.0006 (sh-NC + NC and sh-lnc-CTHCC + NC); P = 0.0005 (sh-lnc-CTHCC + NC and sh-lnc-CTHCC + YAP). For A-F and I-J, data are from three biologically independent experiments and shown as mean ± SEM. Two-tailed paired t-test was performed. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.
