Extended Data Fig. 9: METTL3 promotes cell proliferation and metastasis in HCC.
(a-c) The knockdown and overexpression efficiencies of METTL3 at the RNA and protein levels in Hep3B cells. (A) P = 0.0003. (B) P = 0.0013. (C) The results are representative of three independent experiments. (d-f) Knockdown of METTL3 inhibited cell proliferation and metastasis while overexpression of METTL3 promoted cell proliferation and metastasis in Hep3B cells. (D) P values are as follows: P = 0.0009 (sh-METTL3); P = 0.0012 (Lv-METTL3). (E) Scale bars = 50 μm. P values are as follows: P < 0.0001 (sh-METTL3); P = 0.0010 (Lv-METTL3). (F) Scale bars = 50 μm. P values are as follows: P = 0.0020 (sh-METTL3); P = 0.0004 (Lv-METTL3). (g,h) Knockdown of lnc-CTHCC rescued the proliferation and invasion abilities of Hep3B cells with METTL3 overexpression. (G) P values are as follows: P = 0.0003 (NC + sh-NC and METTL3 + sh-NC); P = 0.0009 (METTL3 + sh-NC and METTL3 + sh-lnc-CTHCC). (H) Scale bars = 50 μm. P values are as follows: P = 0.0022(NC + sh-NC and METTL3 + sh-NC); P = 0.0003 (METTL3 + sh-NC and METTL3 + sh-lnc-CTHCC). For A-B and D-H, data are from three biologically independent experiments and shown as mean ± SEM. Two-tailed paired t-test was performed. **P < 0.01; ***P < 0.001; ****P < 0.0001.
