Extended Data Fig. 6: Functional characterization of MAT2A knockdown in human DMG patients.
A. Quantitative Western Blotting of histone modifications (H3K4me3 and H3K36me3), and SDMA in BT-245 cells comparing MAT2A knockdown to control cells. B. Quantification of histone modifications (H3K4me3 and H3K36me3), SDMA, and MAT2A using Li-Cor fluorescent system for BT-245 cells, MAT2A knockdown vs control cells. Experiments performed in biological replicate of n=3, samples were repeatedly measured 3 times. Statistical analysis performed as two-tailed, unpaired T-test. Data displayed as mean ± s.e.m. (No Dox vs. Dox H3K4me3 **p=0.004557, H3K36me3 **p=0.003499, MAT2A ****p=0.00004, SDMA ***p=0.000578). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. C. Quantitative Western Blotting of histone modifications (H3K4me3, H3K36me3), and SDMA in DIPG04 cells comparing MAT2A knockdown to control cells. D. Quantification of histone modifications (H3K4me3 and H3K36me3), SDMA, and MAT2A using Li-Cor fluorescent system for DIPG04 cells, MAT2A knockdown vs. control cells. Experiments performed in biological replicate of n=3, samples were repeatedly measured 3 times. Statistical analysis performed as two-tailed, unpaired T-test. Data displayed as mean ± s.e.m. (No Dox vs. Dox H3K4me3 ***p=0.000861, H3K36me3 ***p=0.000296, MAT2A **p=0.003325, SDMA **p=0.002049). *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
