Extended Data Fig. 1: Generating primary and orthotopic SCLC with metastases with genome edited lung organoids in mice.
(a) Representative bright-field and fluorescent images of lung organoids transduced with V2TC-sgRNAs and Myc. Three independent repeats. Scale bar, 200 μm. (b) T7 endonuclease 1 (T7E1) assays on Rb1 or Trp53 using infected lung organoid. Cleaved bands were pointed by arrowheads. Three independent repeats. (c) Representative IHC staining of KI67 of lung sections of the PRM mice (n = 4 mice). Scale bar, 20 µm. (d) Representative bright-field and fluorescent images showing metastatic lesions in the lymph nodes of the PRM mice (n = 4 mice). Scale bar, 2 mm. (e) Representative H&E staining of the lymph nodes (n = 4 mice). Scale bar, 20 µm. (f) Representative TTF1 staining of the lymph nodes (n = 4 mice). Scale bar, 20 µm. (g) Representative pictures showing staining of ASCL1, CHGA, SYP and NEUROD1 of liver sections of the PRM mice (n = 4 mice). Scale bar, 40 µm. (h) Representative pictures showing staining of ASCL1, CHGA, SYP and NEUROD1 of the PRM mice’s lymph node (n = 4 mice). Scale bar, 40 µm.
