Extended Data Fig. 1: Limited loss of methylation in B-ALL.

a) Genome browser tracks for WGBS data of representative precursor B cells and B-ALL subtypes for an exemplary locus (ACER1 and neighboring genes; chr19:6,282,123-6,425,048, same as in Fig. 1b). B-ALL subtypes exhibit only mild loss of global methylation compared to their healthy counterpart. b) Correlation of CpG methylation levels between precursor B cells and B-ALL subtypes (blue = low density, red = high density, same samples as in Fig. 1b). Black lines mark the difference of 0.1 from the diagonal in both directions. c) Global methylation levels averaged across all covered CpGs outside of CGIs per sample for T-ALL samples split by sex and age. Lines denote the median, edges denote the IQR, whiskers denote 1.5 × IQR and minima/maxima are represented by dots. The number of independent samples is indicated at the top. No significant differences were detected between the groups (two-sided Wilcoxon rank-sum test).