Extended Data Fig. 5: LIPA is the target of ERX-41. | Nature Cancer

Extended Data Fig. 5: LIPA is the target of ERX-41.

From: Targeting LIPA independent of its lipase activity is a therapeutic strategy in solid tumors via induction of endoplasmic reticulum stress

Extended Data Fig. 5

a-c: Dose response curve of the effect of ERX-41 on the proliferation of parental MDA-MB-436 cells and two MDA-MB-436 clones with LIPA KO by CellTiter-Glo assays (a). RT-qPCR evaluation of the effect of 1μM ERX-41 on expression of sXBP1 (b) and DDIT3 (c) in parental MDA-MB-436 cells and MDA-MB-436 clones with LIPA KO is graphed. n = 2 biologically independent samples. d: Effect of ERX-41 on the proliferation of parental SUM-159 cells and SUM-159 cells with LIPA KO stably expressing the ER membrane marker mCherry-RAMP4 as measured by CellTiter-Glo. Data are presented as mean ± SEM. n = 3 biologically independent samples. e: Western blot shows effect of 1μM ERX-41 or thapsigargin or tunicamycin treatment on expression of UPR proteins in parental SUM-159 and SUM-159 cells with LIPA KO. The experiments e were done twice independently with similar results. Numerical source data for a, b, c, d and uncropped blots for e are provided.

Source data

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