Extended Data Fig. 8: LIPA is a viable molecular target in other solid tumors.
a: Dose response curves of parental and LIPA KO clones of ERα + MCF-7 cells show effect of increasing concentrations of ERX-41 at 48 h after treatment. Data are presented as mean ± SEM, n = 3 biologically independent experiments. b: Evaluation of the effect of 1μM ERX-41 on the induction of p-PERK (up shifted band) in parental and LIPA KO clones of ERα + MCF-7 cells. c-e: CTG assays showing the dose response curves for the effect of ERX-41 on cell viability of a number of glioblastoma (c), pancreatic (d) and ovarian (e) cancer cell lines in vitro. Data are presented as mean ± SEM, n = 3 biogically independent experiments. f-j: ES2/GFP/LUC cells were injected i.p. and tumors established. Mice (n = 3) were treated with vehicle or ERX-41 (10 mg/kg/day/i.p). Tumor volume (f), tumor picture (g), tumor weight (h), number of tumor nodules (i) and ascites volume (j) are shown. Data are presented as mean ± SEM, n = 3 mice per group. Significance was determined by unpaired two tailed Student’s t-test. The experiments in b were done twice independently with similar results. Numerical source data for a, c, d, e, f, h, i, j and uncropped blots for b are provided.
