Fig. 2: ERX-41 has potency against TNBC.
a, Following establishment of s.c. MDA-MB-231 xenografts, 10 mg kg–1 single-dose ERX-41 was administered either PO or i.p. Tumor was harvested at 0, 0.5, 1.5, 3, 6 and 24 h after drug administration, and drug levels assayed by LC–MS/MS and graphed. Data presented as mean ± s.e.m.; i.p. group, n = 3 mice; PO group, n = 2 mice. b–e, Following establishment of MDA-MB-231 xenografts in mammary fat pad, daily administration of 10 mg kg–1 ERX-41 or vehicle control was initiated. Tumor volumes were measured using digital calipers and graphed (b). Tumor weights (c), body weights (d) and extirpated tumors (e) at study end are also shown. Data shown as mean ± s.e.m. For tumor volume, n = 10; significance was determined by two-way ANOVA with Bonferroni’s multiple comparisons test. Adjusted P values of last time points are shown. For tumor weight (n = 10) and body weight (n = 5), significance was determined by unpaired two-tailed Student’s t-test. f–h, After establishment of D2A1 syngeneic xenografts in mammary fat pad, daily administration of 10 mg kg–1 ERX-41 or vehicle control was initiated. Tumor volumes were graphed (f), along with tumor weights (g) and body weights (h). Data shown as mean ± s.e.m. For tumor volume data, n = 8; significance was determined by two-way ANOVA with Bonferroni’s multiple comparisons test. Adjusted P values of last time points are shown. For tumor weight (n = 8) and body weight (n = 5), significance was determined by unpaired two-tailed Student’s t-test. i–p, Effect of ERX-41 on growth (i,k,m,o) and tumor weight (j,l,n,p) in four TNBC-PDXs compared with vehicle. Data presented as mean ± s.e.m.; TNBC-PDX-1 (i,j), n = 6; TNBC-PDX-89 (k,l), n = 7; TNBC-PDX-96 (m,n), n = 8; TNBC-PDX-98 (o,p), n = 6 mice. For tumor volume data, significance was determined by two-way ANOVA with Bonferroni’s multiple comparisons test. Adjusted P values of last time points are shown. For tumor weight, significance was determined by unpaired two-tailed Student’s t-test. This experiment was performed once. Numerical source data for a–d,f–p are provided.
