Extended Data Fig. 1: Frozen RMS patient samples have similar cell states as those in PDX models. | Nature Cancer

Extended Data Fig. 1: Frozen RMS patient samples have similar cell states as those in PDX models.

From: Single-cell analysis and functional characterization uncover the stem cell hierarchies and developmental origins of rhabdomyosarcoma

Extended Data Fig. 1

a,b, UMAP showing all cells sequenced from representative FN-RMS 20696. Non-tumor cells were assigned using Cellassign and clusterprofiler enricher analysis (a, n = 1,726 cells) and tumor cells analyzed for expression of diagnostic markers for rhabdomyosarcoma (b, n = 1,676 cells). In total, 3,402 cells were analyzed in this experiment. c, UMAP visualization of tumor cells from primary FN-RMS 20696 (n = 1,676 cells). d, Heatmap showing single cells (x axis) and genes enriched for specific transcription modules (y axis, FN-RMS 20696). Cells are arranged by UMAP clusters, combined based on expression similarity, and then assigned a specific cell state as noted. Cell numbers same as panel c. e, UMAP renderings for primary RMS samples juxtaposed with graphical analysis showing detected genes/tumor cells when analyzed across different cell states (n = 1,676 cells for sample 20696; n = 9,675 cells for 29806; n = 11,902 cells for 21202; and n = 6,188 cells for 20082).

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