Extended Data Fig. 8: Characterization and functional analysis of CDH11 expression in EL and PT lesions.

(a, b) CDH11 (green) protein expression in MMTV- HER2 (HER2, red) EL and PT cells. Scales, 25 μm. Graph shows n = 3 mice per group, median and two-tailed Mann–Whitney test. (c) CDH11 mRNA expression in MMTV- HER2 EL and PT spheres. Graph shows n = 3, median and two-tailed Mann–Whitney test. (d, e) mRNA expression of CDH11 (D) and 3D-Matrigel invasive phenotype (E) of MMTV-HER2 EL organoids transfected with siControl or siCDH11. Graphs show n = 2 (D) and n = 4 (E), median and two-tailed Mann–Whitney test. (f, g) mRNA expression of CDH11 (F) and 3D-Matrigel invasive phenotype (G) of MMTV-HER2 PT organoids 7 days after CDH11-OE. Graphs show n = 3 (F) and n = 4 (G), median and two-tailed Mann–Whitney test. (h) Representative images of CDH11 (red or gray) protein expression in primary tumors and lung metastasis of mice injected with PT Control and PT CDH11-OE spheres. Scales 20 μm. (i) Tumor volume over time of PT Control and PT CDH11-OE mice, until the primary tumor reached size for surgery. Graph shows n = 6 PT Control and 8 PT CDH11-OE mice, median, interquartile range and two-tailed multiple t-tests.

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