Extended Data Fig. 5: FcγR dependent activation underlies myeloid reprograming following anti-CTLA-4 m2a treatment.
(a) Enrichment of lymphoid cell type frequencies in DT vs control in Foxp3DTR background, or anti-CTLA-4 m2a versus control in WT background. Values are log2 fold change of the mean frequency of each cell type across mice, dot size indicates the average frequency of the cell population between treatments, and color the p-value of two-tailed Student’s t test between each treatment and control. (b) Comparison of the fractions of the indicated lymphoid cell types in individual mice in each treatment group. n = 4 and 5 biologically independent samples for Ctrl and DT groups respectively were used for statistical analysis. (c) As in (a), but for myeloid cells. (d) As in (b), but for myeloid cells. n = 4 and 5 biologically independent samples for Ctrl and DT groups respectively were used for statistical analysis. (e) Enrichment of lymphoid cell type frequencies in anti-CTLA-4 m2a versus anti-CTLA-4 m1 in FCGR KO background, or anti-CTLA-4 m2a versus anti-CTLA-4 m1 in WT background. Values are log2 fold change of the mean frequency of each cell type across mice, dot size indicates the average frequency of the cell population between treatments, and color the p-value of two-tailed Student’s t test between each treatment and control. (f) Comparison of the fractions of the indicated lymphoid cell types in individual mice in each treatment group. n = 7 and 4 biologically independent samples for m1 and m2a groups respectively were used for statistical analysis. (g) As in (e), but for myeloid cells. (h) As in (f), but for myeloid cells. n = 6 and 6 biologically independent samples for m1 and m2a groups respectively were used for statistical analysis. In (b), (d), (f), and (h), Bars represent mean ± SE of mice per treatment arm. Two-tailed Student’s t test was used.
