Extended Data Fig. 1: Flow cytometry gating strategy and scRNAseq data pre-processing steps. | Nature Cancer

Extended Data Fig. 1: Flow cytometry gating strategy and scRNAseq data pre-processing steps.

From: Anti-CTLA-4 antibodies drive myeloid activation and reprogram the tumor microenvironment through FcγR engagement and type I interferon signaling

Extended Data Fig. 1

(a) Gating strategy used to sort CD45+ cells or TCRb+ (T) cells. (b) Violin plot depicting the distribution of detected genes per cell per sample. (c) Violin plot depicting the distribution of unique molecular identifiers (UMIs) per cell per sample. (d) Violin plot depicting the distribution of percentage of UMIs coming from mitochondrial genes per cell per sample. (e) Explained variance (Y) as function of principal components (PCs, X) for PCA of normalized expression values of all cells and 3,000 most variable genes (Methods). UMAP of all cells before (left) and after (right) batch correction by CCA (Methods).

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